This project pioneers a novel 3D spatial proteomics platform for chronic diseases by integrating Lund University’s advanced bioimaging (light-sheet microscopy/MERFISH) with Aarhus University’s multiplexed imaging-powered Deep Visual Proteomics (mipDVP). Our cross-border collaboration develops an innovative workflow combining:
- high-plex 3D tissue imaging,
- AI-guided single-cell analysis/automated microdissection, and
- ultra-sensitive mass spectrometry to map protein networks in chronic diseases.
Initially focused on chronic obstructive pulmonary disease (COPD), we will establish the first end-to-end pipeline preserving spatial context during tissue remodeling. In parallel, we demonstrate platform versatility by resolving controversies in human pancreas islet architecture (e.g., true 3D islet size vs. 2D estimates and α-cell deficient islets).
The project leverages Öresund-Kattegat-Skagerrak-region research infrastructures and Miltenyi Biotech’s platforms to create a transferable framework with clinical translation pathways, including immunohistochemistry diagnostic kits for COPD biomarkers and diabetes-relevant islet signatures.
Expected outcomes: validated 3D spatial proteomics protocols; novel microenvironment-specific biomarkers; and strengthened academia-industry collaboration. This project will develop advanced 3D spatial biology tools with direct patient impact across chronic diseases within the HALRIC community.
For further information about this HALRIC pilot project, please contact:
Xiang Zheng
Aarhus University
xiangzheng@biomed.au.dk
