Epicutaneous patch testing is widely used to diagnose allergic contact dermatitis, yet current methods are time-consuming and rely on petrolatum-based formulations that are not optimal for delivering certain test substances.

This HALRIC pilot project explored the use of non-lamellar liquid crystalline lipid systems as multifunctional materials for both delivering allergens to the skin and simultaneously sampling endogenous biomarkers in a non-invasive manner.

The project developed new glycerol monooleate-based formulations capable of accommodating and releasing both hydrophilic and lipophilic substances more efficiently than traditional patch test matrices. Laboratory studies demonstrated significantly enhanced release of clinically relevant allergens such as nickel and chromium, indicating the potential to shorten patch testing duration.

Clinical investigations further showed that these formulations can simultaneously deliver test compounds and collect skin metabolites, enabling a combined diagnostic approach. Metabolomic analysis (LC–MS) revealed changes in natural moisturising factor components following allergen exposure, highlighting the potential to identify biomarkers associated with allergic reactions and distinguish them from irritant responses.

By combining formulation development, clinical validation and advanced metabolomics, the project establishes a promising platform for faster, more accurate and patient-friendly skin allergy diagnostics. The work also lays the foundation for future development of non-invasive, personalised diagnostic tools.